State-of-the art microbiological diagnosis 5 of bacteria relies on phenotyping characterization via cultivation on chromogenic media 6 in combination with DNA detection (PCR) 7 or mass spectrometry (MS) approaches 8. Additionally, tailored and personalized treatment pathways and antibiotic stewardship becomes increasingly important to reduce infection rates in hospitals and save lives and resources 3, 4. One approach to counteract infections is their early detection and therefore there is a great need for fast and specific diagnostic tools. Often no or only limited diagnostic tools are available and treatment options are vanishing due to emerging antibiotic resistances 1, 2. Microbial infections are one of the major causes of death in a global context. This type of multiplexing with NIR fluorescent nanosensors enables remote detection and differentiation of important pathogens and the potential for smart surfaces. Sensors are also spectrally encoded (900 nm, 1000 nm, 1250 nm) to differentiate the two major pathogens P. These hydrogels are exposed to clinical isolates of 6 important bacteria ( Staphylococcus aureus, Escherichia coli,…) and remote (≥25 cm) NIR imaging allows to identify and distinguish bacteria.
They are chemically tailored to detect released metabolites as well as specific virulence factors (lipopolysaccharides, siderophores, DNases, proteases) and integrated into functional hydrogel arrays with 9 different sensors.
The nanosensors are based on single-walled carbon nanotubes (SWCNTs) that fluoresce in the NIR optical tissue transparency window, which offers ultra-low background and high tissue penetration. Here, we developed a set of near infrared (NIR) fluorescent nanosensors and used them for remote fingerprinting of clinically important bacteria. Optimal methods would be non-invasive and without extensive sample-taking/processing.
Fast and specific detection of pathogens such as bacteria is needed to combat these diseases. Infectious diseases are worldwide a major cause of morbidity and mortality.
0 kommentar(er)
